Coding

Part:BBa_K2324001:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-11)


FimH+sfGFP at residue 225


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.

References